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Updated: Apr 12, 2026
Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
Published on: August 4, 2021
Galectin-1 reduction and changes in T regulatory cells may play crucial roles in patients with unexplained recurrent
Minjun Wu1, Ping Liu1, Licun Cheng1
1Shanghai Changning District Obstetrics Maternal and Child Health Hospital Shanghai 200051, China.
To investigate the changes of Galectin-1 and T-lymphocyte phenotypes in unexplained recurrent spontaneous abortion (URSA). Totally 60 participants were recruited and divided into 3 groups in average: pregnant patients with URSA (URSA group), normal early pregnant women with induced abortion (IA group) and normal non-pregnant women (control group). After the tissue and blood sample were collected, Galectin-1 was measured using enzyme-linked immunosorbent assay. Then the proportion of T regulatory cells was determined by flow cytometry. The expression levels of Galectin-1 in IA group and URSA group was significantly higher than that in the control group (24.30 ± 3.06 and 6.23 ± 2.41 vs. 1.30 ± 0.66, P < 0.05). Besides, the expression level of Galectin-1 in URSA group was lower than that in IA group (P < 0.05). The percentage of CD4(+)CD25(+)Foxp3(+) Tregs was lower in URSA group than IA group (0.77 ± 0.31 vs. 1.00 ± 0.35, P < 0.05) and the ratio of CD4(+)CD25(+)Foxp3(+)/CD4(+) in URSA group was also obviously lower than that in IA and control group (P < 0.05). Galectin-1 and CD4(+)CD25(+)Foxp3(+) may play essential roles in maintaining a normal pregnancy and their reduction may involve in the pathogenesis of URSA.
To investigate the changes of Galectin-1 and T-lymphocyte phenotypes in unexplained recurrent spontaneous abortion (URSA). Totally 60 participants were recruited and divided into 3 groups in average: pregnant patients with URSA (URSA group), normal early pregnant women with induced abortion (IA group) and normal non-pregnant women (control group). After the tissue and blood sample were collected, Galectin-1 was measured using enzyme-linked immunosorbent assay. Then the proportion of T regulatory cells was determined by flow cytometry. The expression levels of Galectin-1 in IA group and URSA group was significantly higher than that in the control group (24.30 ± 3.06 and 6.23 ± 2.41 vs. 1.30 ± 0.66, P < 0.05). Besides, the expression level of Galectin-1 in URSA group was lower than that in IA group (P < 0.05). The percentage of CD4(+)CD25(+)Foxp3(+) Tregs was lower in URSA group than IA group (0.77 ± 0.31 vs. 1.00 ± 0.35, P < 0.05) and the ratio of CD4(+)CD25(+)Foxp3(+)/CD4(+) in URSA group was also obviously lower than that in IA and control group (P < 0.05). Galectin-1 and CD4(+)CD25(+)Foxp3(+) may play essential roles in maintaining a normal pregnancy and their reduction may involve in the pathogenesis of URSA.
