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Updated: Apr 12, 2026
Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
Published on: August 28, 2018
Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus
1Key Laboratory of Experimental Marine Biology, National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, Shandong, People's Republic of China.
Abstract:
A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.
