Related Experiment Video
Updated: Apr 12, 2026

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
Screening of a microbial consortium for highly simultaneous degradation of lignocellulose and chlorophenols
Jiajin Liang1, Xiang Peng2, Dexing Yin2
1College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, PR China.
Abstract:
In this work, spent mushroom substrates were utilized for screening a microbial consortium with highly simultaneous degradation of lignocellulose and chlorophenols. The desired microbial consortium OEM1 was gained through successive cultivation for about 50 generations and its stability of composition was verified by denaturing gradient gel electrophoresis (DGGE) during screening process. It could degrade lignocellulose and chlorophenols at around 50% and 100%, respectively, within 7days. The diversity analysis and the growth characteristics of OEM1 during degradation process were investigated by PCR-DGGE combined with clone and sequence. The results indicated that OEM1 consisted of 31 strains. Proteobacteria and Bacteroidetes were the predominant bacterial groups. The dynamic change of OEM1 illustrated that consortium community structure was effected by pH and substrate alteration and tended to be stable after 6days' cultivation. Furthermore, bacteria (11 strains) and actinomycetes (2 strains) were obtained based on plate isolation and identified via 16S rDNA sequence.
More Related Videos
16:33Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
10:21A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Published on: February 1, 2011
Related Concept Videos
Microbial Bioremediation of Pesticides
Microbial Bioremediation of Hydrocarbons