Influence of monosaccharides on the fibrillogenesis of type I collagen
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A N Rathi1, S Padmavathi, G Chandrakasan
1Biological Sciences Laboratory, Central Leather Research Institute, Madras, India.
Biochemical Medicine and Metabolic Biology
|December 1, 1989
Abstract:
The in vitro self-assembly of Type I rat tail tendon collagen in the presence of monosaccharides was studied turbidimetrically. D(+)glucose and D(+)mannose delayed fibril formation significantly while D(-)fructose showed no variation. The effect of glucose was concentration dependent. Binding of glucose during fibrillogenesis was shown by the incorporation of [14C]glucose and also by SDS-polyacrylamide slab gel electrophoresis of the product. The results indicate that in vitro nonenzymatic glycosylation can take place in the short duration required for fibril formation.