Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

8.9K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.9K
Issues And Trends In Healthcare Delivery System01:29

Issues And Trends In Healthcare Delivery System

6.5K
The issues and trends in healthcare delivery are constantly changing. The COVID-19 pandemic is one recent issue that wreaked havoc on healthcare systems, causing a shortage of healthcare workers, high demand for medicines and supplies, and increased medical expenditure due to a lack of insurance. Other issues include rising healthcare costs and care fragmentation.
Cost Containment
Payment for healthcare services has historically promoted adoption of costly and often unnecessary or inefficient...
6.5K
Hybridoma Technology01:31

Hybridoma Technology

18.7K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
18.7K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.7K
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

671
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
671
DNA Microarrays02:34

DNA Microarrays

23.1K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
23.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine Learning-Enhanced Ultrasensitive Immuno-CRISPR Array Facilitates Early Diagnosis of Alzheimer's Disease by Detecting Multiple Plasma Biomarkers.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Dendritic mesoporous silica nanoparticle-based nasal delivery carriers for passive immunotherapy of Alzheimer's disease.

Neural regeneration research·2026
Same author

HAAC Ligation-Enabled Hierarchical Assembly: Deciphering the Synergy of α-Hydroxyl Aldehydes and 2-Aminooxazoles.

Journal of the American Chemical Society·2026
Same author

Light-Triggered Graphene/Black Phosphorus Heterostructure FET Platform for Ultrasensitive Detection of Alzheimer's Disease Biomarkers at the Zeptomole Level.

Research (Washington, D.C.)·2025
Same author

Mn<sub>3</sub>O<sub>4</sub> nanozyme-based anti-inflammatory therapy modulates microglial phenotype by downregulating TLR4/NOX2 expression and further alleviates Alzheimer's disease pathology.

Theranostics·2025
Same author

A Fault-Tolerant Localization Method for 5G/INS Based on Variational Bayesian Strong Tracking Fusion Filtering with Multilevel Fault Detection.

Sensors (Basel, Switzerland)·2025
Same journal

Iron Single Atom Nanozyme-Mediated GPX4 Inhibitor Delivery for Self-Enhanced Ferroptosis.

Bioconjugate chemistry·2026
Same journal

SpyCatcher-Engineered Ferritin Nanocages Enable Dual-Receptor Targeting for Enhanced Glioma Therapy.

Bioconjugate chemistry·2026
Same journal

One-Pot Synthesis of Functionalized Coumarin Fluorophores Enables Rapid Access to Live-Cell Bioorthogonal Labeling and Microenvironmental Sensing Agents.

Bioconjugate chemistry·2026
Same journal

Enzymatic Synthesis of Glyco-DNA Equipped with Oligosaccharides and Charged Monosaccharides.

Bioconjugate chemistry·2026
Same journal

Electrochemical Tryptophan-Selective Bioconjugation in Neutral Buffer via Cooperative <i>N</i>-Oxyl Radicals.

Bioconjugate chemistry·2026
Same journal

Conjugates of Heme and Intrinsically Disordered Peptides toward the Development of Synthetic Artificial Oxygen Carriers.

Bioconjugate chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 12, 2026

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
11:16

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

37.8K

HaloTag technology: a versatile platform for biomedical applications.

Christopher G England1, Haiming Luo2, Weibo Cai1,2,3

  • 1†Department of Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.

Bioconjugate Chemistry
|May 15, 2015
PubMed
Summary
This summary is machine-generated.

The HaloTag protein fusion platform offers a versatile solution for studying protein function and interactions, overcoming limitations of previous methods. This innovative technology enables comprehensive analysis with a single genetic construct for diverse biomedical applications.

More Related Videos

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

17.6K
The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.7K

Related Experiment Videos

Last Updated: Apr 12, 2026

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
11:16

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

37.8K
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

17.6K
The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.7K

Area of Science:

  • Proteomics
  • Molecular Biology
  • Biomedical Research

Background:

  • Protein function and interaction studies are vital for understanding genomics, proteomics, and disease.
  • Current methods using affinity and autofluorescent tags have limitations in specificity and require complex genetic constructs.
  • The complexity of biological proteomes challenges comprehensive protein analysis.

Purpose of the Study:

  • To review the applications of the HaloTag protein fusion platform in biomedical research.
  • To highlight the advantages of HaloTag technology over traditional protein analysis methods.
  • To discuss current and potential future uses of HaloTag for studying protein behavior.

Main Methods:

  • Utilizing the HaloTag protein fusion platform with a single genetic construct.
  • Employing specific HaloTag ligands for high-affinity and specific binding to the HaloTag protein.
  • Applying the technology across various research areas including protein isolation, function, interactions, and imaging.

Main Results:

  • HaloTag technology enables comprehensive analysis of protein function and interactions.
  • The platform simplifies protein analysis by requiring only a single genetic construct.
  • HaloTag demonstrates high affinity and specificity in binding, enhancing experimental reliability.

Conclusions:

  • The HaloTag platform provides a powerful and versatile tool for diverse biomedical applications.
  • It overcomes limitations of existing protein analysis techniques, offering enhanced specificity and ease of use.
  • Future applications of HaloTag technology hold significant promise for advancing biological and medical research.