Related Experiment Video

Updated: Apr 12, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.4K

[Antibiotic susceptibility and identification of clinical Pseudomonas fulva isolates]

E P Sivolodsky, G V Gorelova, S P Bogoslovskaya

    Antibiotiki I Khimioterapiia = Antibiotics and Chemoterapy [Sic]
    |May 16, 2015
    PubMed

    Insights

    The earliest clinical strains of Pseudomonas fulva were identified, confirming its medical importance. Methods for routine identification without genetic analysis were approved, and antibiotic resistance was noted.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Bacterial Identification

    Context:

    • Pseudomonas fulva is an emerging opportunistic pathogen.
    • Clinical strains were isolated in St. Petersburg between 1995 and 2005.
    • The medical significance of P. fulva necessitates reliable identification methods.

    Purpose:

    • To confirm the medical importance of Pseudomonas fulva.
    • To evaluate MALDI-TOF mass spectrometry for P. fulva identification.
    • To characterize the antibiotic susceptibility profile of clinical P. fulva strains.

    Summary:

    • Eight early clinical strains of Pseudomonas fulva were identified, confirming the species' medical relevance.
    • MALDI-TOF mass spectrometry using the MALDI Biotyper system demonstrated high accuracy for P. fulva identification.
    • Routine identification methods not requiring genetic analysis were validated.
    • The antibiotic susceptibility profile revealed acquired resistance in two isolates to 3rd generation cephalosporins and chloramphenicol.

    Impact:

    • Establishes Pseudomonas fulva as a clinically significant bacterium.
    • Validates MALDI-TOF MS as a rapid and accurate method for P. fulva identification in clinical settings.
    • Provides crucial data on antibiotic resistance patterns, informing treatment strategies.
    • Supports the development of non-genetic diagnostic tools for P. fulva.

    More Related Videos

    Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung07:46

    Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

    Published on: June 5, 2012

    34.3K
    Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control05:09

    Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control

    Published on: March 15, 2024

    6.7K

    Related Concept Videos

    Clinical Significance of Antibiotic Resistance01:25

    Clinical Significance of Antibiotic Resistance

    Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
    54
    Mechanism of Antibiotic Resistance in MRSA01:25

    Mechanism of Antibiotic Resistance in MRSA

    Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
    132
    Gene Regulation in Microbial Communities: Quorum Sensing01:28

    Gene Regulation in Microbial Communities: Quorum Sensing

    Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
    913
    Antibiotic Selection00:57

    Antibiotic Selection

    Overview
    62.2K