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Updated: Apr 12, 2026

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Published on: September 28, 2018
Mechanistic Aspects of Folded Protein Transport by the Twin Arginine Translocase (Tat)
1From the Plant Molecular and Cellular Biology, University of Florida, Gainesville, Florida 32611 kcline@ufl.edu.
Abstract:
The twin arginine translocase (Tat) transports folded proteins of widely varying size across ionically tight membranes with only 2-3 components of machinery and the proton motive force. Tat operates by a cycle in which the receptor complex combines with the pore-forming component to assemble a new translocase for each substrate. Recent data on component and substrate organization in the receptor complex and on the structure of the pore complex inform models for translocase assembly and translocation. A translocation mechanism involving local transient bilayer rupture is discussed.
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