Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The workflow from post-mortem human brain sampling to cell microdissection: a Brain Net Europe study.

David Meyronet1, Aline Dorey, Patrick Massoma

  • 1INSERM U1028; CNRS UM5292; Lyon Neuroscience Research Center, Neuro-oncology and Neuro-inflammation Team, 69000, Lyon, France, david.meyronet@chu-lyon.fr.

Journal of Neural Transmission (Vienna, Austria : 1996)
|May 16, 2015
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intensified Rituximab, Methotrexate, Procarbazine, and Cytarabine (R-MPVA) Regimen With Sequential Addition of Etoposide to R-MPV and Ifosfamide to R-AraC for Newly Diagnosed Primary CNS Lymphoma Patients Aged Between 60 and 70 Years: An LOC Network Study.

Hematological oncology·2026
Same author

How modern imaging can change diagnoses in historical cases of constitutional bone diseases.

Archives of disease in childhood·2026
Same author

Magnetic Resonance Imaging Features in Intramedullary Tumors: A Pictorial Review.

Biomedicines·2026
Same author

A quest for a histaminergic or orexinergic biomarker for sudden infant death syndrome.

Pediatric research·2026
Same author

COQ7-Related Neuropathy: Two New Cases and Review of the Literature.

Journal of the peripheral nervous system : JPNS·2026
Same author

Glial fibrillary acidic protein (GFAP) in biofluids: analytical considerations and clinical relevance in neurodegenerative diseases.

Journal of neurology·2026
This summary is machine-generated.

Brain banks can use laser capture microdissection for disease research. Optimizing the entire process from autopsy to analysis is crucial for preserving tissue quality for molecular studies.

Area of Science:

  • Neuroscience
  • Pathology
  • Molecular Biology

Background:

  • Brain banks are essential biological resource centers for storing characterized brain tissue.
  • Advancements in molecular analysis techniques (nucleic acids, proteins) enable cell-level disease research.
  • Laser capture microdissection (LCM) offers potential for precise tissue analysis.

Purpose of the Study:

  • To evaluate and establish a workflow for LCM compatible with brain bank constraints.
  • To assess tissue processing from sampling to pre-analytical quality control.
  • To optimize methods for preserving morphological and molecular quality of brain tissue.

Main Methods:

  • Comparison of various brain tissue freezing techniques.
  • Adaptation of staining protocols to reduce neuronal autofluorescence.

Related Experiment Videos

  • Evaluation of nucleic acid and protein yields post-processing.

    • Main Results:

    • Laser capture microdissection is feasible within brain bank settings.
    • Freezing methods and staining protocols impact morphological and molecular quality.
    • The overall process affects protein and nucleic acid integrity, limiting sample availability.

    Conclusions:

    • A comprehensive workflow from autopsy to analysis is necessary for successful LCM in brain banks.
    • Careful consideration of tissue processing is vital to mitigate impacts on molecular quality.
    • LCM holds promise for cell-level neuropathology research using banked brain tissue.