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Proton nuclear magnetic resonance methyl and methylene linewidths from plasma decrease during postprandial lipemia.

R B Verdery1, D F Benham, I McLennan

  • 1Bowman Gray School of Medicine, Section on Internal Medicine and Gerontology, Winston-Salem, NC 27103.

Biochimica Et Biophysica Acta
|December 18, 1989
PubMed
Summary

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Fasting and postprandial lipemia affect plasma proton nuclear magnetic resonance (1H-NMR) linewidths. Postprandial lipemia can narrow 1H-NMR linewidths, mimicking findings previously associated with malignancy.

Area of Science:

  • Biochemistry
  • Medical Diagnostics
  • Nuclear Magnetic Resonance Spectroscopy

Background:

  • Narrow proton nuclear magnetic resonance (1H-NMR) linewidths in plasma have been linked to malignancy.
  • Previous studies did not control for fasting status, a potential confounding factor.

Purpose of the Study:

  • To investigate the impact of postprandial lipemia on plasma 1H-NMR linewidths in healthy individuals.
  • To determine the relationship between plasma lipoprotein levels and 1H-NMR linewidths in fasting and postprandial states.

Main Methods:

  • Measured 1H-NMR methyl and methylene linewidths in plasma from normolipemic, non-cancer individuals.
  • Collected plasma samples during fasting and at 90-minute intervals after a fat meal.
  • Quantified plasma lipoprotein levels to assess postprandial lipemia.

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Main Results:

  • Plasma 1H-NMR linewidths (methyl, methylene, average) positively correlated with high-density lipoprotein (HDL) levels.
  • Linewidths inversely correlated with triacylglycerol-rich lipoprotein (TRL) levels.
  • Linewidths decreased postprandially, reaching minimums at peak plasma triacylglycerol levels.

Conclusions:

  • Postprandial lipemia significantly narrows plasma 1H-NMR linewidths.
  • This narrowing can be comparable to linewidths observed in subjects with cancer.
  • Fasting status is a critical consideration when interpreting plasma 1H-NMR linewidths for malignancy detection.