Related Experiment Video
Updated: Apr 12, 2026

15:22
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
17.9K
A procedure for the preparation and isolation of nucleoside-5'-diphosphates
Heidi J Korhonen1, Hannah L Bolt2, David R W Hodgson2
1Department of Chemistry, Science Laboratories, Durham University, South Road, Durham, DH1 3LE, United Kingdom, ; Department of Chemistry, University of Turku, Vatselankatu 2, 20014 Turku, Finland.
Beilstein Journal of Organic Chemistry
|May 16, 2015
Abstract:
Tris[bis(triphenylphosphoranylidene)ammonium] pyrophosphate (PPN pyrophosphate) was used in the SN2 displacements of the tosylate ion from 5'-tosylnucleosides to afford nucleoside-5'-diphosphates. Selective precipitation permitted the direct isolation of nucleoside-5'-diphosphates from crude reaction mixtures.

