Related Experiment Video
Updated: Apr 12, 2026

Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica
Published on: January 16, 2026
Artificial selection on anther exsertion in wild radish, Raphanus raphanistrum
Jeffrey K Conner1, Cynthia J Mills2, Vanessa A Koelling3
1Kellogg Biological Station and Department of Plant Biology, Michigan State University , 3700 East Gull Lake Drive, Hickory Corners, Michigan 49060, USA ; National Evolutionary Synthesis Center , 2024 W. Main St, Durham, North Carolina 27705, USA.
Abstract:
To study the genetic architecture of anther exsertion, a trait under stabilizing selection in wild radish, artificial selection on anther exsertion was applied for 11 generations. Two replicate lines each of increased and decreased exsertion plus two randomly-mated controls were included. Full pedigree information is available from generation five. To estimate correlated responses to selection, 571 plants from all lines and matrilines were grown in the greenhouse and a number of floral, growth, and phenology traits were measured. To create an outbred F2 mapping population, all possible crosses among the two high and two low exsertion lines were made, using a multiple-family design to capture the genetic variance still present after 11 generations of selection. Six floral traits were measured on 40 parents, 240 F1, and 4,868 F2 offspring. Opportunities for reuse of these data include traits not previously analyzed, other analyses, especially using the pedigree and fitness data, and seeds from all generations and photos of flowers in the later generations are available.
More Related Videos
Related Concept Videos
Plant Breeding and Biotechnology
Asexual Reproduction
Frequency-dependent Selection
Types of Selection

