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Updated: Apr 12, 2026
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Exopolysaccharide assay in Escherichia coli microcolonies using a cleavable fusion protein of GFP-labeled
Yoshihiro Ojima1, Asep Suparman1, Minh Hong Nguyen1
1Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Abstract:
A fused protein composed of a carbohydrate-binding module and green fluorescence protein (GFP) was developed to measure the exopolysaccharides (EPShs) present in Escherichia coli microcolonies. The cleavage of the GFP part of this protein using a site-specific protease allowed for the non-invasive and quantitative evaluation of the EPShs.
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