Ultra-sensitive high performance liquid chromatography-laser-induced fluorescence based proteomics for clinical...

Area of Science:

• Proteomics
• Biochemistry
• Analytical Chemistry

Background:

• Proteomics techniques are advancing for disease mechanism understanding.
• Existing methods include HPLC, 2D-gel electrophoresis, and mass spectrometry.

Purpose of the Study:

• To develop an ultra-sensitive HPLC-LIF technique for cancer screening and early detection.
• To utilize protein profiling of clinical samples for cancer staging.

Main Methods:

• Developed an ultra-sensitive high performance liquid chromatography-laser induced fluorescence (HPLC-LIF) technique.
• Analyzed over 300 protein profiles from clinical samples (serum, saliva, tissue).
• Employed principal component analysis (PCA) for objective classification of conditions.

Main Results:

• Achieved objective detection and classification of malignant, premalignant, and healthy conditions.
• Demonstrated high sensitivity with a limit of detection in the femto-mole range.
• Successfully applied HPLC-LIF and PCA for oral and cervical cancer diagnosis.

Conclusions:

• The developed HPLC-LIF combined with PCA is a sensitive proteomic method for cancer screening and diagnosis.
• This technique shows potential for routine use in early cancer detection and staging.
• The study contributes to the field of proteomics in India.