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A needle-free technique for interstitial fluid sample acquisition using a lorentz-force actuated jet injector
Jean H Chang1, N Catherine Hogan1, Ian W Hunter1
1BioInstrumentation Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 3-147, Cambridge, MA 02139, USA.
Summary
A new needle-free jet injector rapidly collects dermal interstitial fluid (ISF) samples. This method shows promise for faster ISF acquisition compared to traditional techniques.
Area of Science:
- Biomedical Engineering
- Fluid Dynamics
- Dermal Physiology
Background:
- Dermal interstitial fluid (ISF) sampling is crucial for diagnostics.
- Conventional methods can be invasive and time-consuming.
- Needle-free technologies offer potential advantages for sample acquisition.
Purpose of the Study:
- To develop and validate a novel, rapid method for acquiring dermal interstitial fluid (ISF) samples.
- To investigate the parameters influencing ISF extraction efficiency and dilution.
- To assess the feasibility of this method in both ex vivo and in vivo models.
Main Methods:
- Utilized a Lorentz-force actuated needle-free jet injector for ISF sampling.
- Employed a two-step process: initial saline injection followed by negative pressure for ISF extraction.
- Investigated injection/extraction parameters and developed a finite element model for analysis.
- Validated the method on post-mortem porcine tissue and subsequently in live rats.
Main Results:
- Successfully acquired ISF samples from post-mortem porcine skin (up to 3.5% ISF in 3.1s).
- Demonstrated feasibility in live rats, extracting samples with a dilution factor of 111-125.
- Finite element model supported faster extraction times compared to conventional methods.
Conclusions:
- The Lorentz-force actuated jet injector provides a rapid method for dermal ISF acquisition.
- The developed technique shows potential for minimally invasive and efficient ISF sampling.
- Further optimization is needed to reduce sample dilution in live subjects.
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