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Potyviruses depend on target of rapamycin (TOR) signaling for infection. Inhibiting TOR severely impacted watermelon mosaic virus (WMV) but only slightly delayed turnip mosaic virus (TuMV), indicating differing viral needs.

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Virology

Background:

  • Potyviruses are significant plant pathogens.
  • Target of rapamycin (TOR) signaling regulates essential eukaryotic cellular processes like translation and autophagy.
  • Plant viruses often hijack host cellular machinery for replication.

Purpose of the Study:

  • To investigate the dependence of potyviruses on active plant TOR signaling.
  • To determine if different potyviruses have distinct requirements for TOR signaling.
  • To explore potential antiviral strategies targeting TOR signaling.

Main Methods:

  • Utilized RNA interference (RNAi) to downregulate TOR in Arabidopsis.
  • Infected Arabidopsis with watermelon mosaic virus (WMV) and turnip mosaic virus (TuMV).
  • Applied AZD-8055, a TOR kinase inhibitor, to infected plants.

Main Results:

  • Downregulation of TOR severely altered WMV accumulation.
  • TuMV accumulation was only slightly delayed in TOR-downregulated lines.
  • AZD-8055 strongly inhibited WMV infection and could cure existing infections.
  • TuMV infection was unaffected by AZD-8055 treatment.

Conclusions:

  • Potyviruses exhibit differential dependence on active plant TOR signaling.
  • WMV appears highly reliant on TOR, while TuMV shows less dependence.
  • Targeting TOR signaling presents a potential strategy for controlling specific potyvirus infections.