[Application research of protein test by using biuret reagent]

Area of Science:

• Clinical Chemistry
• Biochemistry

Background:

• The biuret assay is a common method for protein quantification.
• Variability in test results can arise from different assay components.

Purpose of the Study:

• To evaluate the impact of different instruments, reagents, and calibrators on protein detection using the biuret reagent.
• To determine the sources of bias in biuret protein assays.

Main Methods:

• Twenty-seven distinct detection systems were created by combining three instruments, reagents, and calibrators.
• Each system analyzed five serum samples, with outliers removed to calculate mean AST values.
• Bias for each system was calculated to assess the influence of different components.

Main Results:

• Detection equipment did not significantly impact biuret reagent bias (P = 0.421).
• Different detection reagents showed a statistically significant impact on biuret reagent bias (P = 0.011).
• Calibrators significantly affected biuret reagent bias (P = 0.000).

Conclusions:

• Both detection reagents and calibrators significantly influence protein detection results using the biuret reagent.
• These factors must be considered when interpreting biuret assay results to minimize deviation.