Rapid and efficient differentiation of Yersinia species using high-resolution melting analysis
Roberto A Souza1, Miliane R Frazão1, Alzira M P Almeida2
1Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, SP, Brazil.
Journal of Microbiological Methods
|May 19, 2015
Abstract:
The primary goal of clinical microbiology is the accurate identification of the causative agent of the disease. Here, we describe a method for differentiation between Yersinia species using PCR-HRMA. The results revealed species-specific melting profiles. The herein developed assay can be used as an effective method to differentiate Yersinia species.
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