Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling MARCH6's role in cancer progression and metabolism from protein homeostasis to oncogenesis.

Pharmacological researchĀ·2026
Same author

Bioorthogonal Iridium(III) Complexes: A New Frontier of Luminescent Iridium(III) Complexes for Biomedical Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)Ā·2026
Same author

Dual-Receptor Targeted Imaging of Cancer Cells with a Bioorthogonal Iridium(III)-Based Probe.

Inorganic chemistryĀ·2026
Same author

Dissecting Mitochondrial Sulfur Dioxide Generation Mechanism in Rheumatoid Arthritis with a NIR Luminogenic Iridium(III)-Based Probe.

Analytical chemistryĀ·2026
Same author

Imaging mitochondrial hydrogen sulfide in multicellular spheroids using a near-infrared iridium(III) complex.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopyĀ·2026
Same author

Facilitating Quantitation of Mitochondrial G-Quadruplex DNA with an Iridium(III) Two-Photon Phosphorescence Lifetime Imaging Probe.

Journal of the American Chemical SocietyĀ·2025

Related Experiment Video

Updated: Apr 12, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K

Recent Development of G-Quadruplex Probes for Cellular Imaging.

Dik-Lung Ma1, Modi Wang, Sheng Lin

  • 1Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. edmondma@hkbu.edu.hk.

Current Topics in Medicinal Chemistry
|May 19, 2015
PubMed
Summary

G-quadruplexes are DNA structures found in the genome, potentially regulating genes and causing disease. This review focuses on new probes for detecting these structures in living cells.

More Related Videos

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.8K

Related Experiment Videos

Last Updated: Apr 12, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.8K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • G-quadruplexes are non-canonical DNA structures formed by guanine-rich sequences.
  • These structures are prevalent in human genome regions like telomeres and oncogene promoters.
  • G-quadruplexes are implicated in gene regulation and diseases, including those related to telomere maintenance.

Purpose of the Study:

  • To review recent advancements in G-quadruplex probe development over the past three years.
  • To highlight methods for detecting and imaging G-quadruplexes within living cells.
  • To discuss the biological relevance and detection of RNA G-quadruplexes.

Main Methods:

  • Bioinformatics analysis of guanine-rich sequences.
  • Development of specific molecular probes.
  • Cellular imaging techniques for G-quadruplex detection.

Main Results:

  • G-quadruplexes are widespread in the human genome, particularly in functionally important regions.
  • Specific probes have been developed for G-quadruplex detection and imaging.
  • Recent studies emphasize in vivo detection and imaging within living cells.

Conclusions:

  • G-quadruplexes are significant structures with roles in gene regulation and disease.
  • Advanced probes are crucial for understanding G-quadruplex function in biological systems.
  • Further research is needed to fully elucidate the in vivo roles of G-quadruplexes, including RNA forms.