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Updated: Apr 12, 2026

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
Published on: February 5, 2021
Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9
Scott J Gratz1, Melissa M Harrison, Jill Wildonger
1Genetics Training Program, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Abstract:
The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach.
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