Antimicrobial activity and cytotoxicity of nanofibrous mats immobilized with polysaccharides-rectorite based nanogels
Jianwei Zhang1, Xueyong Li2, Jing Tian3
1Department of Environmental Science, Hubei Key Lab of Biomass Resource and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Abstract:
Rectorite (REC)-encapsulated lysozyme (LY)-alginate (ALG) nanogels (NGs) were prepared by adding ALG-REC composites suspensions into LY solutions at the mass ratio of 1:2. The morphology of the NGs and the NGs-assembled nanofibrous mats were studied by transmission electron microscope and field emission scanning electron microscopy, respectively. The composition of NGs-immobilized nanofibrous mats was detected by X-ray photoelectron spectroscopy. The NGs-assembled nanofibrous mats with the addition of REC could enhance the inhibition against Escherichia coli and Staphylococcus aureus. Additionally, NGs-coated mats reduced the toxicity of cellulose mats on mouse lung fibroblasts using MTT assay. Besides, the addition of REC in the NGs improved the cell compatibility of NGs-assembled nanofibrous mats.
