DNA nanostructures based biosensor for the determination of aromatic compounds

Area of Science:

• Electrochemistry
• Nanotechnology
• Biochemistry

Background:

• Electrochemical detection of aromatic compounds is crucial for environmental and biological monitoring.
• Developing sensitive and selective biosensors remains a key challenge.
• Nanomaterials offer unique properties for enhancing biosensor performance.

Purpose of the Study:

• To develop a novel electrochemical biosensor for detecting aromatic compounds.
• To utilize DNA nanostructures (nsDNA) for improved electrode modification.
• To investigate the interaction mechanism between nsDNA and aromatic compounds.

Main Methods:

• Modification of graphite electrodes with multi-walled carbon nanotubes (MWCNT), chitosan (CS), glutaraldehyde (GTA), and nsDNA.
• Characterization of the modified electrode using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).
• Electrochemical detection of aromatic compounds using Electrochemical Impedance Spectroscopy (EIS), Differential Pulse Voltammetry (DPV), and Cyclic Voltammetry (CV).

Main Results:

• The nsDNA-modified electrode demonstrated an effective platform for electron transfer.
• Electrochemical Impedance Spectroscopy revealed non-covalent binding between nsDNA and aromatic compounds.
• The nsDNA-based biosensor exhibited high selectivity and sensitivity in identifying aromatic compounds.

Conclusions:

• The nsDNA-modified electrode is a viable platform for electrochemical sensing.
• Non-covalent interactions play a dominant role in the detection mechanism.
• The developed biosensor shows promise for selective and sensitive detection of aromatic compounds.