Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bone Formation by Intramembranous Ossification01:29

Bone Formation by Intramembranous Ossification

17.4K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
17.4K
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

15.7K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
15.7K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

4.8K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.8K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

3.8K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dental public health in action: Oral health messaging and the smoking cessation team.

Community dental health·2026
Same author

Resolving trabecular metaphyseal bone profiles downstream of the growth plate adds value to bone histomorphometry in mouse models.

Frontiers in endocrinology·2023
Same author

Targeting osteocytes vs osteoblasts.

Bone·2023
Same author

Uniparental disomy screen of Irish rare disorder cohort unmasks homozygous variants of clinical significance in the <i>TMCO1</i> and <i>PRKRA</i> genes.

Frontiers in genetics·2022
Same author

Regional variations in discrete collagen fibre mechanics within intact intervertebral disc resolved using synchrotron computed tomography and digital volume correlation.

Acta biomaterialia·2021
Same author

Life course longitudinal growth and risk of knee osteoarthritis at age 53 years: evidence from the 1946 British birth cohort study.

Osteoarthritis and cartilage·2020

Related Experiment Video

Updated: Apr 12, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

8.5K

MMP and TIMP temporal gene expression during osteocytogenesis.

M Prideaux1, K A Staines2, E R Jones3

  • 1The University of Adelaide, North Terrace, Adelaide, SA 5005, Australia.

Gene Expression Patterns : GEP
|May 19, 2015
PubMed
Summary

Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) regulate osteocyte differentiation. Specific MMPs and TIMPs are linked to extracellular matrix remodeling essential for osteocyte development.

Keywords:
MMPMineralisationOsteoblastOsteocyteTIMP

More Related Videos

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
10:48

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Published on: November 13, 2023

1.7K
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
07:23

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Published on: December 3, 2016

12.6K

Related Experiment Videos

Last Updated: Apr 12, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

8.5K
IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
10:48

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Published on: November 13, 2023

1.7K
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
07:23

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Published on: December 3, 2016

12.6K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Bone Biology

Background:

  • Osteocytes are crucial for bone health, differentiating from osteoblast precursors within the mineralized extracellular matrix (ECM).
  • The molecular mechanisms governing osteocyte differentiation, particularly the role of ECM-remodeling enzymes, remain largely unknown.
  • Matrix metalloproteinases (MMPs) are key ECM remodelers, but their function during osteocytogenesis is undefined.

Purpose of the Study:

  • To investigate the temporal gene expression of MMPs and their inhibitors, tissue inhibitors of metalloproteinases (TIMPs), during osteocytogenesis.
  • To determine the role of specific MMPs and TIMPs in regulating the differentiation of osteocytes.

Main Methods:

  • Utilized MLO-A5 cells, a cell line model for osteocyte differentiation.
  • Employed quantitative reverse transcription polymerase chain reaction (RT-qPCR) to analyze MMP and TIMP gene expression.
  • Manipulated mineralization conditions to assess the link between MMP/TIMP expression and osteocyte differentiation.

Main Results:

  • Identified expression of 14 MMPs and 3 TIMPs in MLO-A5 cells.
  • Observed significant changes in MMP2, MMP23, MMP28, MMP14, and MMP19 mRNA levels during mineralisation.
  • Found differential expression of TIMP1, TIMP2, and TIMP3 mRNA correlated with mineralisation status, linking MMPs to osteocyte differentiation.

Conclusions:

  • Several MMP and TIMP family members are identified as key regulators of ECM remodeling during osteocytogenesis.
  • These MMPs and TIMPs are essential for acquiring the osteocyte phenotype.
  • The study provides novel insights into the molecular control of osteocyte differentiation.