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Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
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Developing a common bean core collection suitable for association mapping studies
Juliana Morini Küpper Cardoso Perseguini1, Gliciane Micaele Borges Silva2, João Ricardo Bachega Feijó Rosa3
1Departamento de Genética e Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil . ; Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Campinas, SP, Brazil .
Genetics and Molecular Biology
|May 19, 2015
Summary
Researchers developed a core collection of 180 common bean accessions from 500 to efficiently conserve genetic variability. This curated set maximizes the representation of genetic diversity for breeding and future studies.
Area of Science:
- Plant genetics
- Crop science
- Agricultural research
Background:
- Large germplasm collections pose challenges for exploring genetic diversity.
- Efficient conservation and breeding require representative core collections.
Purpose of the Study:
- To quantify and analyze genetic variability in 500 common bean accessions.
- To construct a core collection for conservation and breeding.
Main Methods:
- Utilized 58 SSR markers to genotype 500 common bean accessions.
- Employed Structure analysis and Analysis of Molecular Variance (AMOVA).
- Selected 180 accessions for the core collection based on genetic diversity.
Main Results:
- Polymorphism Information Content (PIC) ranged from 0.17-0.86; Discriminatory Power (DP) ranged from 0.21-0.90.
- Structure analysis identified 15 groups in the base collection and 4 groups in the core collection.
- The 180 selected accessions captured 97.2% of the total genetic variability.
Conclusions:
- The constructed core collection efficiently represents the genetic diversity of the base collection.
- This core collection is valuable for association mapping and directing breeding programs.
- Facilitates the development of elite common bean cultivars for global market needs.
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