Creatinine determination according to Jaffe-what does it stand for?

Area of Science:

• Clinical Chemistry
• Analytical Chemistry

Background:

• The Jaffe reaction, discovered in 1886, is a foundational method for creatinine determination.
• Despite newer enzymatic methods, the Jaffe assay persists due to its cost-effectiveness and ease of use.
• Historical development traces back to Max Jaffe's observation and Otto Folin's analytical principles.

Purpose of the Study:

• To highlight the enduring relevance of the Jaffe method for creatinine analysis.
• To address the persistent issue of methodological variations and lack of standardization in the Jaffe assay.
• To emphasize the ongoing need for improved interchangeability of Jaffe results in modern diagnostics.

Main Methods:

• The Jaffe method involves the reaction of creatinine with picric acid in an alkaline medium.
• The original work described precipitate formation, not a quantitative assay.
• Methodological variations have evolved over time, leading to a lack of a standardized protocol.

Main Results:

• The Jaffe reaction's fundamental principles remain central to creatinine determination.
• Significant methodological diversity exists in current Jaffe assay implementations.
• Lack of standardization compromises the comparability of creatinine measurements across different laboratories.

Conclusions:

• The Jaffe method's simplicity and low cost ensure its continued use in creatinine analysis.
• Methodological inconsistencies present a significant challenge for accurate and interchangeable Jaffe results.
• Further efforts are needed to standardize the Jaffe assay for reliable clinical interpretation.