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Updated: Apr 12, 2026
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
36.4K
Applications of CRISPR-Cas9 mediated genome engineering
1State Key Laboratory of Proteomics, Collaborative Innovation Center for Cardiovascular Disorders, Genetic Laboratory of Development and Disease, Institute of Biotechnology, Beijing, 100071 China.
Military Medical Research
|May 19, 2015
Summary
The CRISPR-Cas9 system, a revolutionary genome engineering tool, simplifies creating animal models and treating diseases. Its applications span basic research to human gene therapy, transforming biomedical studies.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Homologous recombination has been vital for studying biological mechanisms and disease.
- Genome engineering technologies are rapidly advancing biological and medical research.
Purpose of the Study:
- To highlight the impact of CRISPR-Cas9 on biological and medical research.
- To discuss the broad applications and future potential of CRISPR-Cas9 technology.
Main Methods:
- Utilizing RNA-guided endonucleases, specifically the CRISPR-Cas9 system.
- Leveraging homologous recombination for targeted mutagenesis.
Main Results:
- The CRISPR-Cas9 system offers simplicity and widespread applicability.
- This technology facilitates germline animal model generation, somatic genome engineering, and functional genomic screening.
Conclusions:
- CRISPR-Cas9 is revolutionizing biology and medical studies.
- The technology's potential extends across all biomedical fields, including basic research and human gene therapy.
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