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Related Experiment Videos

Immunoreactive anionic and cationic trypsin in human serum.

M Kimland1, C Russick, W H Marks

  • 1Yale University School of Medicine, New Haven CT.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|September 15, 1989
PubMed
Summary
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This study presents a new purification method for human trypsinogen and trypsin using affinity chromatography. It also details ELISA assays for trypsin-like immunoreactivity, showing increased levels in acute pancreatitis and uremia.

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Immunology

Background:

  • Trypsinogen and trypsin are crucial pancreatic enzymes.
  • Accurate measurement of trypsinogen and trypsin in serum is important for diagnosing pancreatic diseases.
  • Existing methods for purification and quantification may be insufficient.

Purpose of the Study:

  • To develop a simple and effective method for purifying anionic and cationic trypsinogen and trypsin from human pancreatic juice.
  • To establish enzyme-linked immunoabsorbent assay (ELISA) methods for quantifying anionic and cationic trypsin-like immunoreactivity (irAT and irCT) in serum.
  • To determine normal serum levels and ratios of irAT and irCT and investigate their changes in acute pancreatitis and uremia.

Main Methods:

  • Affinity chromatography using aprotinin coupled Sepharose for enzyme purification.

Related Experiment Videos

  • Development and validation of ELISA assays for irAT and irCT.
  • Measurement of serum irAT and irCT levels in healthy individuals, patients with acute pancreatitis, and uremic patients.

    • Main Results:

    • A simple affinity chromatography method successfully purified anionic and cationic trypsinogen and trypsin.
    • Normal serum levels were established: irAT at 21.3 +/- 7.4 microgram/l and irCT at 27.8 +/- 9.0 microgram/l, with an irCT/irAT ratio of 1.36 +/- 0.42.
    • Serum irAT and irCT levels significantly increased in acute pancreatitis and uremic patients, with a proportionally greater increase in irAT.

    Conclusions:

    • The described affinity chromatography and ELISA methods provide efficient tools for trypsinogen and trypsin purification and quantification.
    • Serum trypsin-like immunoreactivity, primarily trypsinogen in normal individuals, serves as a potential biomarker for pancreatic conditions.
    • Elevated irAT and irCT, particularly irAT, in acute pancreatitis and uremia warrant further investigation for diagnostic and prognostic value.