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A microfabrication-free nanoliter droplet array for nucleic acid detection combined with isothermal amplification
Xiaodong Ma1, Weiwei Xu, Chao Chen
1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, P. R. China. zhlu@seu.edu.cn.
The Analyst
|May 20, 2015
Abstract:
A nanoliter droplet array based on a hydrophilic-hydrophobic patterned chip is developed without using microfabrication technology. Combined with the isothermal amplification technology, it has been applied to perform nucleic acid detection, showing excellent specificity and sensitivity. As a versatile platform, it is used to detect three gene targets successfully.
