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Related Concept Videos

Upstream Processing01:27

Upstream Processing

85
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
85
Scale-Up Processes01:14

Scale-Up Processes

88
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
88
iChip01:24

iChip

77
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
77
Batch vs Continuous Culture01:14

Batch vs Continuous Culture

156
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
156

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Related Experiment Video

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High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process

Pascal Baumann1, Tobias Hahn1, Jürgen Hubbuch2

  • 1Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Science, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.

Biotechnology and Bioengineering
|May 20, 2015
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Summary

This study combines high-throughput micro-scale cultivation and in silico chromatography modeling to optimize bioprocesses. The approach balances upstream and downstream performance for superior overall process efficiency.

Keywords:
BioLectorUV absorption modelinghigh-throughput screeningion exchange chromatographymicro-scale cultivation

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Protein Expression

Background:

  • Upstream bioprocess design is complex and cell productivity is difficult to predict.
  • High-throughput cultivation screenings in micro-scale formats are crucial for exploring design spaces.
  • In silico models exist for downstream processes, reducing time and costs.

Purpose of the Study:

  • To present a combined optimization approach for upstream and downstream bioprocesses.
  • To integrate high-throughput micro-scale cultivation with chromatography modeling.
  • To achieve superior overall process performance, not just high product titers.

Main Methods:

  • Utilized high-throughput cultivations in a BioLector micro-scale system (48-well format).
  • Employed in silico chromatography modeling (ChromX) for downstream process optimization.
  • Validated in silico-optimized operational modes for product capturing.

Main Results:

  • Demonstrated a combined optimization strategy for bioprocesses.
  • Successfully applied Cherry-tagged Glutathione-S-Transferase from Escherichia coli SE1 as a case study.
  • Identified optimal upstream and downstream conditions for enhanced process performance.

Conclusions:

  • The integrated approach optimizes both upstream and downstream bioprocess stages.
  • This methodology leads to superior overall process performance beyond maximizing product titers alone.
  • The case study validates the effectiveness of combining experimental and in silico methods for bioprocess optimization.