Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

6.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
6.2K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.6K
Autophagic Cell Death01:18

Autophagic Cell Death

5.1K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
5.1K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

10.4K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
10.4K
Lysosomes01:31

Lysosomes

27.3K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
27.3K
Lysosomes01:31

Lysosomes

3.8K
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the Association between Age, Sex and d-Amino Acid-Based Estimation of Glomerular Filtration Rate.

Clinical journal of the American Society of Nephrology : CJASN·2026
Same author

STING is a key driver of Japanese encephalitis virus induced inflammatory response.

Communications biology·2026
Same author

Unique molecular profiling of monocyte responses to a high dose of DENV-NS1 reflected the effect of NS1 on hemostasis.

Medical microbiology and immunology·2026
Same author

A role for CASM in the repair of damaged Golgi architecture.

Autophagy·2026
Same author

Characteristics of newly diagnosed systemic lupus erythematosus patients with or without kidney involvement: analysis of the National Database of Designated Intractable Diseases of Japan.

Clinical and experimental nephrology·2026
Same author

Epidemiological analysis of kidney involvement in systemic lupus erythematosus using the national database of designated intractable diseases of Japan: a cross-sectional study of renewal applicants.

Clinical and experimental nephrology·2026

Related Experiment Video

Updated: Apr 12, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K

Secretory autophagy.

Marisa Ponpuak1, Michael A Mandell2, Tomonori Kimura2

  • 1Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 915 Camino de Salud NE, Albuquerque, NM 87131, USA.

Current Opinion in Cell Biology
|May 20, 2015
PubMed
Summary
This summary is machine-generated.

Autophagy machinery mediates non-degradative secretory autophagy, enabling unconventional protein secretion. This process exports cytosolic cargo, including leaderless proteins, independent of the endoplasmic reticulum and Golgi apparatus.

More Related Videos

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

18.8K
Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
11:53

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Published on: March 31, 2023

1.9K

Related Experiment Videos

Last Updated: Apr 12, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K
The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

18.8K
Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
11:53

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Published on: March 31, 2023

1.9K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Autophagy traditionally viewed as a degradative process.
  • Emerging non-degradative roles of autophagy are being uncovered.
  • Conventional secretion via ER/Golgi pathway is well-established.

Purpose of the Study:

  • To elucidate the non-degradative functions of autophagy.
  • To investigate the mechanism of secretory autophagy.
  • To understand how autophagy impacts conventional secretion.

Main Methods:

  • Investigated autophagic machinery in non-degradative cargo export.
  • Studied unconventional secretion of leaderless cytosolic proteins.
  • Analyzed the interplay between autophagy and conventional secretory pathways.

Main Results:

  • Autophagy mediates secretory autophagy for non-conventional cargo export.
  • Leaderless cytosolic proteins are secreted via secretory autophagy.
  • Autophagy influences constitutive and regulated secretion.
  • Autophagy promotes alternative trafficking of membrane proteins.

Conclusions:

  • Autophagy has critical non-degradative roles, notably secretory autophagy.
  • Secretory autophagy provides a pathway for exporting cytosolic cargo independently of the ER-Golgi.
  • Autophagy is intricately linked with cellular secretion and protein sorting.