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Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
Published on: November 2, 2011
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Direct measurement of DNA-mediated adhesion between lipid bilayers
S F Shimobayashi1, B M Mognetti, L Parolini
1Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Physical Chemistry Chemical Physics : PCCP
|May 21, 2015
Summary
DNA-mediated multivalent interactions on lipid vesicles were studied. A new theory accurately models liposome adhesion and membrane tension, advancing our understanding of these complex biological and synthetic systems.
Area of Science:
- Biophysics
- Soft Matter Physics
- Materials Science
Background:
- Multivalent interactions are crucial in biological systems, mediating cell-cell and cell-substrate adhesion.
- Artificial materials mimicking these interactions use DNA linkers on deformable units like lipid vesicles.
- Understanding these interactions on soft membranes is complex due to coupled mechanics and statistical effects.
Purpose of the Study:
- To quantitatively characterize DNA-mediated multivalent interactions on a single liposome adhering to a supported bilayer.
- To estimate membrane tension induced by DNA-mediated adhesive forces.
- To develop and validate a theoretical model for these interactions.
Main Methods:
- Experimental investigation of DNA-mediated interactions between a single liposome and a supported bilayer.
- Morphological measurements of the liposome.
- In situ Förster Resonant Energy Transfer (FRET) spectroscopy to probe DNA-melting transitions.
- Comparison of experimental data with an analytical theoretical model.
Main Results:
- The study successfully estimated membrane tension caused by DNA-mediated adhesion.
- Morphological and DNA-melting transition data were collected.
- The analytical theory, with minimal fitting, showed good agreement with experimental results.
Conclusions:
- The developed theory provides a quantitative description of DNA-mediated interactions and vesicle deformation.
- The findings confirm the underlying assumptions of the coupled mechanical and statistical model.
- This work advances the understanding of multivalent interactions in soft matter systems.
