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Updated: Apr 12, 2026
Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
Published on: January 7, 2015
A novel and innovative technique of using a disposable syringe and mesh for harvesting fat for structural fat
B C Ashok1, Pradeep Kumar Nagaraj1, Y N Ananteshwar1
1Department of Plastic Surgery, Manipal Hospital, Bengaluru, Karnataka, India.
Background:
Successful restoration of structure and function using autologous free fat grafts has remained elusive. Review of literature shows that various harvesting and preparation techniques have been suggested. The goal of these techniques is to obtain greater adipocyte cell survival and consequently more reliable clinical results.
Materials And Methods:
In our technique, a piece of mesh is kept at one end of the lipoaspiration syringe, which is then connected to the Suction pump. As one syringe fills, it is replaced by another one until the required amount of fat is obtained.
Results:
By using a polypropylene mesh in our technique, we can separate the transfusate from the harvested fat graft during harvesting itself. The fat graft thus obtained is dense and concentrated, with fewer impurities.
Conclusion:
Hence, we recommend our technique as a reliable method for extracting sterile emulsified fat in an economical way.
