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Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

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Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Microorganisms in Medicine and Therapeutics01:29

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Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
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siRNA - Small Interfering RNAs02:30

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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RNA interference-based therapeutics: molecular platforms for infectious diseases.

Sathish Dyawanapelly, Sharwari Bhagwat Ghodke, Ramya Vishwanathan

    Journal of Biomedical Nanotechnology
    |May 21, 2015
    PubMed
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    RNA interference (RNAi) offers vast therapeutic potential for gene regulation and treating diseases. This review explores RNAi-based strategies against intracellular infections, highlighting challenges and future directions for effective treatments.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Therapeutics

    Background:

    • RNA interference (RNAi) is a powerful tool for gene expression analysis and disease amelioration.
    • RNAi mechanisms like siRNA, miRNA, and shRNA mediate post-transcriptional gene silencing in mammals.
    • RNAi therapeutics show promise in preclinical models for cancer, infections, autoimmune, and genetic disorders.

    Purpose of the Study:

    • To review RNAi-based interventions for intracellular infections.
    • To discuss challenges and opportunities in developing RNAi therapies for pathogens.
    • To explore nanocarrier-mediated delivery systems for RNAi therapeutics.

    Main Methods:

    • Review of current literature on RNAi applications in infectious diseases.
    • Analysis of RNAi-based therapies targeting bacterial, fungal, viral, and protozoan infections.
    • Summary of nanocarrier delivery systems for siRNA and shRNA.

    Main Results:

    • RNAi technology is a promising strategy for combating intracellular pathogens.
    • Nanocarrier systems enhance the delivery of RNAi molecules, overcoming bioavailability challenges.
    • Preclinical and clinical studies show progress but also highlight significant challenges.

    Conclusions:

    • RNAi holds significant therapeutic promise for intracellular infections.
    • Overcoming delivery challenges and addressing preclinical/clinical hurdles are crucial for success.
    • Future perspectives focus on accelerating the development and commercialization of RNAi-based treatments.