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MiR-191 Regulates Primary Human Fibroblast Proliferation and Directly Targets Multiple Oncogenes

Damon Polioudakis1, Nathan S Abell1, Vishwanath R Iyer1

  • 1Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, United States of America.

Plos One
|May 21, 2015
PubMed

Insights

MicroRNA-191 (miR-191) represses proliferation in human fibroblasts. This study identifies novel proto-oncogene targets of miR-191, revealing its complex role in cell growth.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs (miRNAs) are key regulators in various diseases, including cancers.
  • miR-191 is often considered an oncogene, but its function in primary cell proliferation and its full targetome are not well understood.

Purpose of the Study:

  • To construct a comprehensive, genome-wide miR-191 target profile.
  • To investigate the role of miR-191 in the proliferation of primary human fibroblasts.

Main Methods:

  • RNA-induced silencing complex (RISC) immunoprecipitations.
  • Gene expression profiling.
  • Bioinformatic analysis to identify miR-191 targets.

Main Results:

  • A genome-wide miR-191 target profile was established.
  • miR-191 was found to repress proliferation in primary human fibroblasts.
  • Novel miR-191 targets, including proto-oncogenes CDK9, NOTCH2, and RPS6KA3, were identified.
  • Evidence suggests miR-191 primarily targets the coding sequence (CDS) of its targets.

Conclusions:

  • miR-191 plays a significant role in regulating primary human fibroblast proliferation.
  • The identified miR-191 targets and mechanisms provide new insights into miR-191's function in cellular processes and disease.

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