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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Neurulation

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...

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Related Experiment Video

Updated: Apr 12, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Neural differentiation modulates the vertebrate brain specific splicing program.

Alicia Madgwick¹, Philippe Fort², Peter S Hanson³

¹Institute of Genetic Medicine, Newcastle University, Central Parkway, Newcastle NE1 3BZ, United Kingdom.

|May 21, 2015

Summary

This summary is machine-generated.

A core set of 9 conserved alternative splicing events (ASEs) are specific to the vertebrate brain and crucial for neural development. These brain-specific ASEs are conserved across species, highlighting their fundamental role in neural biology.

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Area of Science:

Neuroscience
Genetics
Molecular Biology

Background:

Alternative splicing patterns typically vary between tissues and species, suggesting limited conserved function in fundamental neural biology.
While most alternative splicing events are species-specific, some may play conserved roles in essential biological processes.

Purpose of the Study:

To investigate the existence and conservation of brain-specific alternative splicing events (ASEs) across different species.
To identify conserved ASEs with potential roles in neural development and function.

Main Methods:

Utilized high-throughput RT-PCR to analyze all annotated simple alternative splicing events (ASEs) in the Reference Sequence Database.
Monitored expression patterns of ASEs in various mouse tissues.
Compared splicing patterns across human, mouse, and zebrafish to identify conserved events.

Main Results:

Identified 93 brain-specific switch-like ASEs that change isoform expression between brain and other tissues.
Discovered that a core set of 9 conserved switch-like ASEs exhibit highly conserved tissue-specific splicing patterns in human, mouse, and zebrafish.
Found these conserved ASEs are often within genes encoding neuronal microtubule network proteins and are dynamically regulated during neural stem cell differentiation and zebrafish development.

Conclusions:

Demonstrates a core set of vertebrate brain-specific alternative splicing events are conserved across species.
These conserved ASEs are associated with neural differentiation and play a fundamental role in neural biology, despite overall species-specific variations in splicing.
Highlights the importance of conserved alternative splicing in the regulation of neuronal development.