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Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

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Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
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Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
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A flow-free droplet-based device for high throughput polymorphic crystallization.

Shih-Mo Yang1, Dapeng Zhang, Wang Chen

  • 1Department of Mechanical and Automation Engineering and Shun Hing Institute of Advanced Engineering, The Chinese University of Hong Kong, Hong Kong. scchen@mae.cuhk.edu.hk.

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Summary
This summary is machine-generated.

A novel droplet-based crystallization method offers precise control over crystal size and form, overcoming limitations of conventional techniques for pharmaceutical applications.

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Area of Science:

  • Materials Science
  • Chemical Engineering
  • Pharmaceutical Science

Background:

  • Conventional crystallization methods like stirring face limitations in control, leading to unstable conditions and broad crystal size distributions.
  • Existing emulsion-based platforms can produce high-quality crystals but are restricted to simple crystal forms.
  • There is a need for advanced crystallization techniques to achieve controlled crystal formation and polymorphism for pharmaceutical development.

Purpose of the Study:

  • To introduce a new flow-free, droplet-based crystallization process for highly controlled crystal formation.
  • To demonstrate the capability of this method for producing uniform crystals and exploring polymorphic crystallization.
  • To investigate the precise control over crystal size, morphology, and polymorphism using droplet microenvironment.

Main Methods:

  • A flow-free, droplet-based process utilizing microwell arrays and surface tension for uniform droplet formation.
  • Degassed polydimethylsiloxane (PDMS) powers saturated solution filling, followed by an oil covering step to control water dissolution.
  • Precise control over droplet size (50-300 μm) and temperature to influence crystallization outcomes.

Main Results:

  • Demonstrated successful crystallization of NaCl and glycine, including polymorphic forms (α and γ) of glycine.
  • Achieved precise control over crystal size with a coefficient of variation (CV) below 5% for spherical agglomerates (SAs).
  • Reported the first-time growth of ellipsoidal crystalline agglomerates of glycine with unique spiral structures (~35 μm screw pitch).

Conclusions:

  • The developed droplet-based method enables reliable fabrication of monodispersed crystals with high uniformity and controlled polymorphism.
  • This technique overcomes limitations of conventional methods, offering enhanced control over crystallization conditions and crystal properties.
  • The process holds significant potential for advancing pharmaceutical formulation and the study of crystal growth mechanisms.