Related Experiment Video

Updated: Apr 12, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

16.3K

Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation

Mélina Celik1, Jérôme Wegnez1, Chantal Griveau1

  • 1Conservatoire Botanique National du Bassin Parisien, UMS 2699 Inventaire et Suivi de la Biodiversité, Muséum National d'Histoire Naturelle, 61 Rue Buffon, 75005 Paris, France.

Applications in Plant Sciences
|May 22, 2015
PubMed
Abstract

Keywords:
Viola elatiorViolaceaeconservationmicrosatellitesnext-generation sequencingpopulation genetics

More Related Videos

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

84.9K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.7K

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
101.9K
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
780.0K
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.6K