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Development of 23 polymorphic microsatellite loci in invasive silver wattle, Acacia dealbata (Fabaceae)
Thomas Guillemaud1, Linda Broadhurst2, Isabelle Legoff1
1Institut national de la recherche agronomique (INRA), Université Nice Sophia Antipolis, Centre national de la recherche scientifique (CNRS), UMR 1355-7254 Institut Sophia Agrobiotech, 06900 Sophia Antipolis, France.
Premise Of The Study:
Microsatellite markers were developed for silver wattle, Acacia dealbata (Fabaceae), which is both an ornamental and an invasive weed species. It is native to southeastern Australia and invasive in Europe, Africa, Asia, and the Americas.
Methods And Results:
The pyrosequencing of a microsatellite-enriched genomic DNA library of A. dealbata produced 33,290 sequences and allowed the isolation of 201 loci with a minimum of seven repeats of microsatellite motifs. Amplification tests led to the setup of two multiplex PCR mixes allowing the amplification of 21 loci. The polymorphism of these markers was evaluated on a sample of 32 individuals collected in southeastern Australia. The number of alleles and the expected heterozygosity varied between two and 11, and between 0.11 and 0.88, respectively.
Conclusions:
The level of polymorphism of this set of 23 microsatellites is large enough to provide valuable information on the genetic structure and the invasion history of A. dealbata.
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