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Updated: Apr 12, 2026
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
Published on: May 2, 2017
Functional behavior and gene expression of magnetic nanoparticle-loaded primary endothelial cells for targeting
Fatema Tuj Zohra1, Mikhail Medved1, Nina Lazareva1
11Department of Surgery, Drexel University College of Medicine, 245 North 15th Street, NCB Suite 7150, Mail Stop 413, Philadelphia, PA 19102, USA.
Aim:
To assess functional competence and gene expression of magnetic nanoparticle (MNP)-loaded primary endothelial cells (ECs) as potential cell-based therapy vectors.
Materials & Methods:
A quantitative tube formation, nitric oxide and adhesion assays were conducted to assess functional potency of the MNP-loaded ECs. A quantitative real-time PCR was used to profile genes in both MNP-loaded at static conditions and in vitro targeted ECs.
Results:
Functional behavior of MNP-loaded and unloaded cells was comparable. MNPs induce expression of genes involved in EC growth and survival, while repress genes involved in coagulation.
Conclusion:
MNPs do not adversely affect cellular function. Gene expression indicates that targeting MNP-loaded ECs to vascular stents may potentially stimulate re-endothelialization of an implant and attenuate neointimal hyperplasia.
