Related Experiment Video
Updated: Apr 12, 2026

Analysis of Fatty Acid Content and Composition in Microalgae
Published on: October 1, 2013
Effect of light intensity on physiological changes, carbon allocation and neutral lipid accumulation in oleaginous
Qiaoning He1, Haijian Yang1, Lei Wu1
1Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Chlorella sp. and Monoraphidium sp. were the potential microalgal species for lipid production. This study aimed to investigate different light intensities (40, 200, 400 μmol photon m(-2) s(-1)) on physiological changes, photosynthetic carbon partitioning and neutral lipid accumulation in both microalgae. Results suggested that under high light (HL, 400 μmol photon m(-2) s(-1)), chlorophyll degraded, protein and carbohydrate content decreased; more carbon allocated into lipid as well as most of intracellular space was occupied by lipid bodies. Moreover, with the lipid accumulation, Fv/Fm decreased and ROS scavenging enzyme increased. Membrane lipid reduced dramatic (29.73-37.97%) to format NL (71.66% of total lipid in Chlorella sp. L1 and 60.65% in Monoraphidium dybowskii Y2). The NL productivity under HL (51.36 and 49.71 mg L(-1) d(-1)) were more than 3 times of those under LL. Additionally, FAME profiles proved that the useful fatty acid components for biodiesel production were enhanced under HL.
More Related Videos
Related Concept Videos
Biofuels
Channel Rhodopsins
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Green Algae
Marine Microbial Ecology
Overview of Algae

