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[Effects of exercise and conjugated linoleic acid on PPARγ in adolescent obese rats]
Objective:
To explore effects of exercise and conjugated linoleic acid (CLA) on PPARy in adolescent obese male SD rats.
Methods:
obese rats were modeled with high fat feeding, 32 obese rats were selected and randomly divided into control group, static + CLA group, exercise-treated group, exercise + CLA treated group. Blood and adipose tissue. were collected after 8 weeks, and blood lipid was measured. PPARγ mRNA gene expression in adipose tissue was tested using qRT-PCR, PPARγ protein expression in adipose tissue by immunohistochemistry, concentration of PPARγ in plasma by ELISA.
Results:
(1) TC level of static + CLA group and exercise group were lower than the control group (P <0. 05). TG level of the exercise group and exercise group + CLA were lower than the control group, static + CLA group (P <0. 01). LDL-c level of exercise group and exercise + CLA group was higher than the control group (P <0. 05), HDL-c level have no difference in groups. (2) PPAR-γ concentration in plasma in exercise group, exercise + CLA group was higher than the control group and static + CLA group (P <0. 01). Expression of PPARy mRNA in adipose tissue in exercise group, exercise + CLA group was higher than the control group and static + CLA group (P <0. 01). PPARγ concentration in plasma and expression of PPARy mRNA in adipose tissue in static + CLA group were higher than control group but with no statistical significance. (3) The situation of PPARγ protein expression was the same with the expression of PPARγ mRNA.
Conclusion:
8 weeks of different intervention methods can reduce the concentration of TG and TC in blood lipid index in adolescent obese rats, TG of exercise and exercise + CLA is better decreasing than simply add CLA. Exercise and exercise + CLA can improve the expression of PPARy mRNA and protein in adipose tissue and plasma PPARγ concentration in rats.
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