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Published on: March 20, 2018
[Establishment a method for identification of the poly(ADP-ribose) binding proteins induced by benzo(a)pyrene]
Objective:
To establish a method for identification of the poly(ADP-ribose) binding proteins induced by benzo (a) pyrene.
Methods:
Poly (ADP-ribose) binding protein were screened by immunoprecipitation assay and further separated by high performance liquid chromatography (HPLC) and two dimensional electrophoresis, then identified by MALDI-TOF-MS/MS. The proteins sequence were identified by two methods and compared the common binding motif with literature reports.
Results:
Three poly (ADP-ribose) binding proteins were identified by MALDI-TOF-MS/MS combined with immunoprecipitation assay and HPLC, and twelve poly (ADP-ribose) binding proteins were identified by MALDI-TOF-MS/MS combined with immunoprecipitation assay and two dimensional electrophoresis. Most of them have a common binding motif which was consistent with the reported.
Conclusion:
Combined the immunoprecipitation assay and two dimensional electrophoresis with MALDI-TOF-MS/MS could be used to analyze the poly(ADP-ribose) binding proteins, and these proteins have a common conserved binding motif.
