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Updated: Apr 12, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC
Published on: March 15, 2017
[Uncertainty evaluation for determining ginsenosides in ginseng food by HPLC]
Objective:
To discuss the influencing factors for uncertainty and evaluate the measurement uncertainty for the determination of ginsenosides Rg1, Re, Rb1 and Rd in ginseng food by HPLC.
Methods:
According to the regulation of JJF1059. 1 - 2012, the components of uncertainty in the measuring process were studied and quantified for the calculation of combined uncertainties and expanded uncertainties.
Results:
The expanded uncertainties for the HPLC determination of ginsenosides Rg1, Re, Rb1 and Rd were 0. 0766, 0. 0700, 0. 0628 and 0. 0722 g/100g (k = 2) respectively from sample of Radix ginseng, and expanded uncertainties of ginsenosides Rg1, Re, Rb1 and Rd were 0. 0476, 0. 0672, 0. 0516 and 0. 0514 g/100g(k = 2) from sample of Red ginseng.
Conclusion:
The uncertainty from pretreatment and standard curve fitting process were the main sources of uncertainty.
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