Related Experiment Video

Updated: Apr 12, 2026

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
10:42

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Published on: December 12, 2017

16.5K

[The application of CRISPR-Cas9 gene editing technology in viral infection diseases]

Li-juan Yin, Si-qi Hu, Fei Guo

    Yi Chuan = Hereditas
    |May 23, 2015
    PubMed

    More Related Videos

    Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing12:21

    Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

    Published on: January 7, 2019

    14.4K
    Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection11:28

    Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

    Published on: May 23, 2016

    18.4K

    Related Concept Videos

    CRISPR/Cas9 Genome Editing01:28

    CRISPR/Cas9 Genome Editing

    The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
    3.1K
    CRISPR01:59

    CRISPR

    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
    60.1K
    CRISPR01:59

    CRISPR

    19.0K
    CRISPR and crRNAs02:53

    CRISPR and crRNAs

    Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
    The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
    20.3K
    The Antiviral System of Bacteria and Archaea: CRISPR01:23

    The Antiviral System of Bacteria and Archaea: CRISPR

    CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
    1.0K
    Homologous Recombination02:31

    Homologous Recombination

    The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
    65.8K