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Target of Rapamycin Complex 2 regulates cell growth via Myc in Drosophila

Ying Kuo1, Huanwei Huang1, Tao Cai1

  • 1National Institute of Biological Sciences, Beijing, China.

Scientific Reports
|May 23, 2015
PubMed

Insights

Target of rapamycin complex 2 (TORC2) and the transcription factor Myc share a common pathway to regulate cell growth. TORC2 influences Myc

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Target of rapamycin (TOR) is a conserved kinase regulating cell growth and metabolism.
  • TOR forms two complexes, TORC1 and TORC2, with distinct functions.
  • TORC2's role in cell growth regulation is not fully understood, unlike TORC1.
  • The transcription factor Myc is crucial for cell proliferation and growth.

Purpose of the Study:

  • To investigate the relationship between TORC2 and Myc in cell growth regulation.
  • To elucidate the molecular mechanisms by which TORC2 controls cell growth.

Main Methods:

  • Genetic analysis of TORC2 components (lst8, rictor) and Myc.
  • Assessment of cell growth defects and rescue experiments.
  • Analysis of Myc's nuclear localization and transcriptional activity.

Main Results:

  • Myc expression rescued growth defects caused by mutations in TORC2 components.
  • Loss of TORC2 impaired Myc's nuclear localization.
  • TORC2 deficiency inhibited Myc-dependent gene transcription.
  • A novel Myc-dependent pathway for TORC2-mediated cell growth was identified.

Conclusions:

  • TORC2 and Myc collaborate in a common pathway to regulate cell growth.
  • TORC2 impacts cell growth by modulating Myc's activity and localization.
  • This study reveals a new mechanism for TORC2 in controlling cellular processes.

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