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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
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Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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Transcription

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Related Experiment Video

Updated: Apr 12, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Primerize: automated primer assembly for transcribing non-coding RNA domains.

Siqi Tian¹, Joseph D Yesselman¹, Pablo Cordero²

¹Departments of Biochemistry, Stanford University, Stanford CA 94305, USA.

Nucleic Acids Research

|May 23, 2015

Summary

Primerize offers a cost-effective solution for designing DNA primers for RNA synthesis. This tool optimizes primer assembly, avoiding mispriming for efficient in vitro transcription and RNA preparation.

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Last Updated: Apr 12, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

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Published on: July 2, 2010

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

Area of Science:

Molecular Biology
Biotechnology
Bioinformatics

Background:

Custom RNA synthesis is crucial for biological and biotechnological research.
Traditional chemical RNA synthesis and purification are expensive and challenging for longer sequences.
Existing methods for designing RNA synthesis primers are complex and lack accessible online tools.

Purpose of the Study:

To develop and present Primerize, a novel online server for efficient primer design.
To provide a cost-effective and user-friendly alternative for preparing RNA molecules up to 300 nucleotides.
To address the limitations of current primer design methods for in vitro transcription.

Main Methods:

Development of an efficient primer design algorithm.
Experimental validation of the algorithm for RNA domains.
Implementation of the algorithm into a web server (Primerize).
Utilizing a pipeline involving DNA template assembly, T7 RNA polymerase in vitro transcription, and kit-based purification.

Main Results:

Primerize provides an efficient algorithm for designing template primers for RNA synthesis.
The server is experimentally tested and validated for RNA domains up to 300 nucleotides.
The Primerize server offers a cost-effective and fast alternative to traditional methods.

Conclusions:

Primerize simplifies and optimizes the design of primers for customized RNA synthesis.
The server facilitates the preparation of RNA molecules, benefiting biological and biotechnological research.
Primerize is freely accessible online, promoting wider adoption and research advancement.