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Related Experiment Videos

Tissue glucose utilization during epinephrine-induced hyperglycemia.

K Mészáros1, C H Lang, D M Hargrove

  • 1Department of Physiology, Louisiana State University Medical Center, New Orleans 70112.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|November 1, 1989
PubMed
Summary

Epinephrine infusion significantly boosts glucose utilization in most rat tissues, excluding the brain. This study validates the 2-deoxyglucose (2-DG) tracer method for measuring glucose uptake during hyperglycemia.

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Area of Science:

  • Physiology
  • Metabolic Research

Background:

  • Epinephrine is known to affect glucose metabolism.
  • Understanding tissue-specific glucose uptake is crucial for metabolic research.

Purpose of the Study:

  • To investigate glucose utilization in individual tissues during epinephrine infusion.
  • To validate the 2-deoxyglucose (2-DG) tracer technique in conditions of hyperglycemia.

Main Methods:

  • In vitro validation of the 2-DG tracer method across varying glucose concentrations.
  • In vivo experiments using chronically catheterized rats infused with epinephrine.
  • Measurement of tissue glucose utilization rates using 2-[14C]DG.

Main Results:

  • The 2-DG tracer method's accuracy was confirmed in vitro, even with high glucose levels.

Related Experiment Videos

  • Epinephrine infusion doubled plasma glucose concentrations in rats.
  • Glucose utilization increased by 40-160% in multiple tissues, including muscles, liver, and kidney, but not the brain.

    • Conclusions:

    • The 2-deoxyglucose (2-DG) method is applicable for measuring glucose utilization during hyperglycemia.
    • Epinephrine significantly enhances glucose uptake in various peripheral tissues.
    • Increased plasma glucose concentration likely drives the elevated glucose utilization observed in response to epinephrine.