Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR

CRISPR

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lineage-restricted dependency on an oncofetal SNHG29-IGF2BP1 RNA axis in acute megakaryoblastic leukemia.

Leukemia·2026

Same author

Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex.

Nature communications·2026

Same author

DLK1 is a GATA1s-driven dependency and therapeutic target in Down syndrome-associated myeloid leukemia.

Blood advances·2026

Same author

First-in-Human Study of IL15-Activated Cytokine-Induced Killer Cells After Allogeneic HCT Shows Durable Remission and Serotherapy-Associated Immune Reconstitution in Leukemia.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026

Same author

Insights into spacer acquisition of the type V-A CRISPR-Cas system of Francisella novicida U112.

Nucleic acids research·2026

Same author

An updated evolutionary classification of CRISPR-Cas systems including rare variants.

Nature microbiology·2025

Search research articles

Related Experiment Video

Updated: Apr 12, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Toward Whole-Transcriptome Editing with CRISPR-Cas9.

Dirk Heckl¹, Emmanuelle Charpentier²

¹Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover 30625, Germany.

|May 23, 2015

Summary

This summary is machine-generated.

CRISPR-Cas9 technology enables precise control over gene expression for biomedical research. Advanced systems offer whole-transcriptome regulation, paving the way for new therapeutic strategies.

More Related Videos

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

Related Experiment Videos

Last Updated: Apr 12, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

Precise regulation of gene expression is crucial for advancing biomedical research and developing novel therapeutics.
Existing technologies offer limited scope for controlling gene activity across the entire genome.

Purpose of the Study:

To explore the potential of CRISPR-Cas9 based transcriptional activator systems for targeted gene regulation.
To highlight advancements in multiplex-compatible systems for genome-scale transcriptional control.

Main Methods:

Utilizing CRISPR-Cas9 technology to develop sophisticated transcriptional activator systems.
Employing genome-scale libraries for multiplexed gene expression control.

Main Results:

Demonstrated the capability of CRISPR-Cas9 systems for highly specific gene targeting.
Showcased multiplex compatibility, enabling simultaneous regulation of multiple genes.
Advanced the field towards achieving comprehensive whole-transcriptome control.

Conclusions:

CRISPR-Cas9 based transcriptional activators represent a powerful tool for precise gene regulation.
These systems significantly enhance the potential for whole-transcriptome control in biomedical applications.
Recent advancements pave the way for innovative research and therapeutic interventions.