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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Related Experiment Video

Updated: Apr 12, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

High-throughput sequencing technologies.

Jason A Reuter¹, Damek V Spacek¹, Michael P Snyder¹

¹Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.

|May 23, 2015

Summary

This summary is machine-generated.

Advancements in DNA sequencing technologies have revolutionized biology and medicine, enabling personal genomics and genomic medicine. This review covers high-throughput platforms, assays, and challenges in current sequencing applications.

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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

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Last Updated: Apr 12, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

The human genome sequence has significantly advanced our understanding of human biology, diversity, and disease.
Rapid progress in DNA sequencing technologies over the last decade has paved the way for personal genomics and genomic medicine.

Purpose of the Study:

To discuss commonly used high-throughput sequencing platforms.
To review the expanding range of sequencing assays.
To highlight challenges in current sequencing platforms and their clinical utility.

Main Methods:

Review of high-throughput sequencing platforms.
Analysis of current sequencing assays.
Discussion of technological challenges and clinical applications.

Main Results:

High-throughput sequencing platforms have become integral to modern biological research.
A diverse array of sequencing assays has been developed, expanding research capabilities.
Significant challenges remain in the widespread clinical application of sequencing technologies.

Conclusions:

DNA sequencing advancements are driving the era of genomic medicine.
Continued innovation in sequencing platforms and assays is crucial.
Addressing current challenges is essential for realizing the full clinical potential of genomics.