Related Experiment Video
Updated: Apr 12, 2026

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci
Published on: June 19, 2017
Whitefly genome expression reveals host-symbiont interaction in amino acid biosynthesis
Santosh Kumar Upadhyay1, Shailesh Sharma2, Harpal Singh3
1Department of Botany, Panjab University, Chandigarh, 160014, India; National Agri-Food Biotechnology Institute, (Department of Biotechnology, Government of India), C-127, Industrial Area, S.A.S. Nagar, Phase 8, Mohali, Punjab, 160071, India.
Background:
Whitefly (Bemisia tabaci) complex is a serious insect pest of several crop plants worldwide. It comprises several morphologically indistinguishable species, however very little is known about their genetic divergence and biosynthetic pathways. In the present study, we performed transcriptome sequencing of Asia 1 species of B. tabaci complex and analyzed the interaction of host-symbiont genes in amino acid biosynthetic pathways.
Methodology/Principal Findings:
We obtained about 83 million reads using Illumina sequencing that assembled into 72716 unitigs. A total of 21129 unitigs were annotated at stringent parameters. Annotated unitigs were mapped to 52847 gene ontology (GO) terms and 131 Kyoto encyclopedia of genes and genomes (KEGG) pathways. Expression analysis of the genes involved in amino acid biosynthesis pathways revealed the complementation between whitefly and its symbiont partner Candidatus Portiera aleyrodidarum. Most of the non-essential amino acids and intermediates of essential amino acid pathways were supplied by the host insect to its symbiont. The symbiont expressed the pathways for the essential amino acids arginine, threonine and tryptophan and the immediate precursors of valine, leucine, isoleucine and phenyl-alanine. High level expression of the amino acid transporters in the whitefly suggested the molecular mechanisms for the exchange of amino acids between the host and the symbiont.
Conclusions/Significance:
Our study provides a comprehensive transcriptome data for Asia 1 species of B. tabaci complex that focusses light on integration of host and symbiont genes in amino acid biosynthesis pathways.
More Related Videos
11:51Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
Published on: April 27, 2018
09:25Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
Related Concept Videos
Amino Acid Biosynthetic Pathways
Biosynthesis in Bacteria
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...
Coordination of Gene Expression Processes in Bacteria
Position-effect Variegation