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Cytoplasmic dynein and early endosome transport

Xin Xiang1, Rongde Qiu, Xuanli Yao

  • 1Department of Biochemistry and Molecular Biology, F. Edward Hébert School of Medicine, The Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA, xin.xiang@usuhs.edu.

Cellular and Molecular Life Sciences : CMLS
|May 24, 2015
PubMed

Insights

Cytoplasmic dynein motors move early endosomes along microtubules in fungal cells. The FTS-Hook-FHIP complex is essential for linking these endosomes to dynein-dynactin for minus-end-directed transport.

Area of Science:

  • Cell Biology
  • Molecular Motors
  • Fungal Biology

Background:

  • Microtubule-based organelle transport is vital for eukaryotic cell function.
  • Cytoplasmic dynein is a key motor protein for minus-end-directed cargo transport.
  • Early endosomes are significant cargoes for dynein in filamentous fungi.

Purpose of the Study:

  • To investigate the molecular mechanisms regulating early endosome transport by dynein in fungal hyphae.
  • To identify the protein complexes involved in linking early endosomes to the dynein motor complex.

Main Methods:

  • Utilized genetic and cell biological approaches in filamentous fungi.
  • Investigated the roles of dynein regulators (LIS1, dynactin) and kinesins (kinesin-3, kinesin-1) in endosome trafficking.
  • Characterized the function of the FTS-Hook-FHIP complex in mediating cargo-motor interactions.

Main Results:

  • Dynein, LIS1, and dynactin are crucial for early endosome movement towards microtubule minus ends.
  • Kinesin-3 and dynein drive bi-directional early endosome transport in fungal hyphae.
  • The FTS-Hook-FHIP complex, particularly Hook and FHIP, is essential for connecting early endosomes to the dynein-dynactin machinery.

Conclusions:

  • The FTS-Hook-FHIP complex acts as a linker between early endosomes and the dynein-dynactin motor complex.
  • Specific components of the FHF complex mediate the interaction with both the cargo (early endosome) and the motor (dynein-dynactin).
  • Understanding these interactions provides insight into the regulation of intracellular transport in fungi.

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